Mononuclear Cells

Hematopoietic stem cells / mononuclear stem cells are isolated from the blood, bone marrow or umbilical cord blood.

The proportion of Hematopoietic stem cells is around 0.01% in bone marrow and 0.001% in blood. They can differentiate in to a variety of specialized cells and have capacity of self renewal. They are programmed in such a way that they self destruct themselves if unneeded or harmful, called apoptosis. It is consider that there are two types of Hematopoietic stem cells, one which can renew themselves for long term and proportion of this kind of cells is around 0.001% in bone marrow. The other types of cells are progenitor or precursor cells which can not renew themselves over a long term. They are immature cells which are precursor to fully differentiated cells of the same type of tissue like blood cells, immune cells, blood vessels, muscle cells, bone etc. and hence bone marrow play a vital role in maintaining health.

At StemCure, we provide services for isolation of mononuclear cells from its various sources and further used for differentiation into specific types of cells. Analytical tests are performed before and after Isolation of mononuclear cells to ensure its quality standards.

Therefore BMNC (bone marrow mononuclear cells) transplantation is used to treat blood cancers and certain blood disorders as approved therapy.
 

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Endothelial Progenitor Cells

EPC

Endothelial progenitor cells can be harvested or/and cultured from bone marrow, peripheral blood or umbilical cord blood.

Endothelial progenitor cells are rare cells that circulate in the blood with the ability to differentiate into endothelial cells, the cells that make up the lining of blood vessels. The process by which blood vessels are born de novo is known as vasculogenesis. Some study suggests that endothelial injury in the absence of sufficient circulating progenitor cells may affect the progression of cardiovascular disease.

At StemCure, we provide services to culture endothelial progenitor cells (EPCs). We are successfully culturing EPCs from BM/ PB samples since 2006. At StemCure we isolate and culture EPCs from Bone marrow or peripheral blood by following GMP / GTP standards and maintaining aseptic conditions. After expansion the cultured cells are confirmed by phenotypic and genotypic confirmation like morphological analysis, surface marker study and gene expression study. In gene expression study, we confirm the expression of marker specific to differentiation of EPCs and also check for other markers. Which are specific for MNC to rule out presence or absence of other types of cells. More over the final product is assess for some of the parentral drug criteria like sterility and endotoxin level detections. Normally the culture procedure takes approximately 7-10 days, than it depends on source sample and number of cells required.


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Mesenchymal Stem Cells

Mesenchymal stromal cells (MSCs) are fibroblast-like plastic-adherent cells that can be isolated from a variety of tissues, such as bone marrow, adipose tissue, skeletal muscle, dental pulp, umbilical cord blood, umbilical cord, peripheral blood, placental membrane and amniotic membrane.


MSC

Mesenchymal stem cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. MSCs have a large capacity for self-renewal while maintaining their multipotency. Mesenchymal stem cells can differentiate into a variety of cell types, like, osteoblasts (bone cells), chondrocytes (cartilage cells) and adipocytes (fat cells) in vivo and in vitro. It can also differentiate in to neuron like cells. Adipose tissue is one of the richest sources of MSC’s when compared to bone marrow, there is more than 500 times more stem cells in 1 gram of fat then 1 gram of aspirated bone marrow. Mesenchymal stem cells divide, and their progeny become committed to a specific and distinctive phenotypic pathway, a lineage with discrete steps and, finally, end-stage cells involved with fabrication of a unique tissue type, e.g., cartilage, bone and many other tissue.

At StemCure we provide services for isolate and culture MSCs from bone marrow, adipose tissue and umbilical cord by following GMP / GTP standards and maintaining aseptic conditions. After expansion the cultured cells are confirmed by phenotypic and genotypic confirmation like morphological analysis, surface marker study and gene expression study. In gene expression study, we confirm the expression of marker specific to differentiation of MSCs and also check for other markers, which are specific for MNC to rule out presence or absence of other types of cells. More over the final product is assess for some of the parentral drug criteria like sterility and endotoxin level detections.  Normally the entire culture procedure takes approximately 20 days to 1 month than it depends on source of cells and number of cells required.



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Chondrocytes

Cartilage biopsy, bone marrow and mesenchymal stem cells are the sources for chondrocytes.

Chondrocytes are an inseparable part of the cartilage connective tissue and is encircled by the intracellular extensive matrix. Chondrocytes facilitate fluid exchange between the gelatinous layers of the cartilage. This exchange provides nutrition to the cartilage and removes waste material from it. The bone marrow stem cells initially form into chondroblasts. When these chondroblasts mature they turn into round shaped chondrocytes. When the cartilage gets damaged due to some reason, chondrocytes replace the damaged tissue and starts healing the damaged area. Many times the wear and tear of cartilage is impossible to be repaired naturally. The instability of knee due to the absence of meniscal tissue on the articulating surface is causing a lot of wear and tear of cartilage. Since the capacity of articulate cartilage to repair is low, the injury to articulate cartilage does not heal quickly and lead to degeneration of cartilage. The transplantation of chondrocytes can effectively repair articular cartilage damage by introducing young chondrocytes. Depending on what type of cartilage a cell is in, it may have a slightly different composition; elastic, hyaline, and fibrocartilage are all unique, designed to meet various needs within the body.

At StemCure we provide services for isolate and culture Chondrocytes from cartilage biopsy by following GMP/GTP standards and maintaining aseptic conditions. Chondrocytes are isolated by enzymatic digestion and cultivated in specific growth media. After expansion the cultured cells are lifted from the tissue culture surface and cell suspension is prepared for transplantation. All necessary test like endotoxins, sterility etc. are performed. before dispatch of the suspension. The entire culture procedure takes approximately 1 month.


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Melanocytes

Malenocytes
Skin biopsy is the source for melanocytes.

Melanocytes are melanin-producing cells located in the bottom layer (the stratum basal) of the skin's epidermis, Melanin is a pigment that is responsible primarily for the color of skin and also protects skin form hazardous UVa and UVb rays by absorbing them. Hence the role of active melanocytes is also important to save the skin from being affected by skin cancer.

Melanocytes are used for the treatment of depigmentation like stable vitiligo.

At StemCure we isolate and culture Melanocyte from autologus skin biopsy by following GMP/GTP standards and maintaining aseptic conditions. A small piece of the patient’s own normal skin from the non-exposed area, usually from the back of the ear lobe or buttocks (which is approximately 1/10th of the diseased patch), is taken by Skin specialist / surgeon / cosmetic surgeon at their facility in the transport media supplied in the kit. The skin sample is digested and melanocytes are isolated and cultured in our special cell culture laboratory using different media. There are two products of the melanocytes for depigmentation, melanocytes and keratinocytes cell suspension and cultured melanocytes suspension. The preference of the product is largely based on the area of the patch and other consideration taken by the clinician.


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Keratinocytes

Skin biopsy is the source for keratinocytes.

Keratinocytes

Keratinocytes are the predominant cell type in the epidermis, the outermost layer of the human skin, constituting 95% of the cells found there.  The primary function of keratinocytes is the formation of a barrier against environmental damage such as pathogens (bacteria, fungi, parasites, viruses) heat, UV radiation and water loss. A number of structural proteins (filaggrin, keratin), enzymes (proteases), lipids and antimicrobial peptides (defensins) contribute to maintain the important barrier function of the skin.


At StemCure we provide services for isolate and culture Keratinocyte from autologus skin biopsy by following GMP/GTP standards and maintaining aseptic conditions For this a small piece of the skin is used. The skin sample is digested and keratinocytes are isolated and cultured in our state of art cell culture laboratory using specified media. Once the desired confluency is achieved, then cells are prepared for transplantation. The duration of the culture process depends upon area to be covered and size of the biopsy.


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